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Citations Search
- Notes: The authors initiated translation on a poly(U) mRNA using biotin-labeled phenylalanine tRNA with isolated ribosomes.-The reaction was allowed to bind to Streptavidin MagneSphere A® Paramagnetic Particles, washed four times and then shaken in buffer to remove the ribosomes.- The isolated rRNA was then extracted with phenol/chloroform, ethanol precipitated and used...
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Citations Search
(0003459) - - Products: Dual-Luciferase ® Reporter Assay System | Dual-Luciferase ® Reporter Assay System 10-Pack | MagneGST™ Glutathione Particles | MagneGST™ Protein Purification System | T N T ® T7 Coupled Wheat Germ Extract System | Transcend™ Chemiluminescent Non-Radioactive Translation Detection System | Transcend™ Colorimetric Non...
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Citations Search
(0003459) - - Products: Dual-Luciferase ® Reporter Assay System | Dual-Luciferase ® Reporter Assay System 10-Pack | MagneGST™ Glutathione Particles | MagneGST™ Protein Purification System | T N T ® T7 Coupled Wheat Germ Extract System | Transcend™ Chemiluminescent Non-Radioactive Translation Detection System | Transcend™ Colorimetric Non...
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Citations Search
- Notes: The authors initiated translation on a poly(U) mRNA using biotin-labeled phenylalanine tRNA with isolated ribosomes.-The reaction was allowed to bind to Streptavidin MagneSphere A® Paramagnetic Particles, washed four times and then shaken in buffer to remove the ribosomes.- The isolated rRNA was then extracted with phenol/chloroform, ethanol precipitated and used...
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Citations Search
- Notes: To understand if protein translation occurs in the nucleus of eukaryotic cells, the researchers in this study labeled proteins in vivo by bathing permeablized cells in a translation mix containing radiochemical, biotin or fluorescently tagged amino acids.-The study found that all three types of label gave similar results.-To fluorescently tag proteins, cells were grown, then...
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Citations Search
(0003459) - - Products: Dual-Luciferase ® Reporter Assay System | Dual-Luciferase ® Reporter Assay System 10-Pack | MagneGST™ Glutathione Particles | MagneGST™ Protein Purification System | T N T ® T7 Coupled Wheat Germ Extract System | Transcend™ Chemiluminescent Non-Radioactive Translation Detection System | Transcend™ Colorimetric Non...
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Citations Search
(0003459) - - Products: Dual-Luciferase ® Reporter Assay System | Dual-Luciferase ® Reporter Assay System 10-Pack | MagneGST™ Glutathione Particles | MagneGST™ Protein Purification System | T N T ® T7 Coupled Wheat Germ Extract System | Transcend™ Chemiluminescent Non-Radioactive Translation Detection System | Transcend™ Colorimetric Non...
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Citations Search
- Notes: The 1736 amino acid (predicted MW of 194kDa) protein ZO-1 was translated in vitro with the TNT A® T7 Coupled Reticulocyte System and the Transcenda„¢ Biotinylated tRNA.
(0000436) - - Products: Rabbit Reticulocyte Lysate System, Nuclease Treated | Transcend™ tRNA Items: 1-17 of 17 - © 2008 -Promega Corporation.
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Citations Search
- Notes: To understand if protein translation occurs in the nucleus of eukaryotic cells, the researchers in this study labeled proteins in vivo by bathing permeablized cells in a translation mix containing radiochemical, biotin or fluorescently tagged amino acids.-The study found that all three types of label gave similar results.-To fluorescently tag proteins, cells were grown, then...
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Transcend Non-Radioactive Translation Detection Systems
This biotinylated lysine is added to the translation reaction as a precharged ε-labeled biotinylated lysine-tRNA complex (Transcend™ tRNA) rather than a free amino acid.
Do not subject the Transcend™ tRNA to more than five freeze-thaw cycles.
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FluoroTect GreenLys in vitro Translation Labeling System
The system is based on a lysine-charged tRNA that is labeled at the ε position of the lysine with the fluorophore BODIPY ® -FL.
All rights reserved.-|- Patents, Trademarks and Product Usage Info -|- Privacy Policy -|- Contact Us Promega Corporation | Customer Service : 608-274-4330 | Technical Service : 608-274-4330 // FluoroTect GreenLys in vitro...
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Genetic Markers in E. coli
Mutants need to be supplemented with succinate or lysine + methionine.
The ribosome-associated ppGpp synthetase I can sense ribosomes with an uncharged tRNA in the A site, a sign of starvation.
1, Glover, D., ed.
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Are proteins produced by an in vitro transcription/translation system using Transcend™ tRNA functional?-eNotes
The Transcend™ tRNA is a precharged ε-labeled biotinylated lysine-tRNA complex that allows nonradioactive detection of proteins produced by in vitro translation?
When using Transcend™ tRNA, approximately 25–33% of lysine residues incorporated into the protein are biotinylated lysines.
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Citations Search
- Notes: The 1736 amino acid (predicted MW of 194kDa) protein ZO-1 was translated in vitro with the TNT A® T7 Coupled Reticulocyte System and the Transcenda„¢ Biotinylated tRNA.
(0000436) - - Products: Rabbit Reticulocyte Lysate System, Nuclease Treated | Transcend™ tRNA Items: 1-17 of 17 - © 2008 -Promega Corporation.
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Citations Search
- Notes: To understand if protein translation occurs in the nucleus of eukaryotic cells, the researchers in this study labeled proteins in vivo by bathing permeablized cells in a translation mix containing radiochemical, biotin or fluorescently tagged amino acids.-The study found that all three types of label gave similar results.-To fluorescently tag proteins, cells were grown, then...
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Promega eNotes | Incite
Cells were permeabilized in a physiological buffer and allowed to extend nascent polypeptides by ~15 residues in the presence of tagged precursor molecules (either [ 3 H]lysine, biotin- or BODIPY-lys-tRNA), the remaining 19 amino acids, aminoacyl-tRNA synthetases, GTP and an energy generating system.
Thus, results with the FluoroTect™ GreenLys tag were essentially...
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Promega eNotes - Feature
Promega Corporation 1 2 3 4 5 Transcend™ Non-Radioactive Translation Detection System To verify this translation system is compatible with non-radioactive detection, linearized Luciferase T7 Control DNA * was titrated into the T N T ® T7 Quick for PCR DNA system in the presence of Transcend™ Biotinylated Lysine tRNA ( Cat.# L5061 ).
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Promega eNotes - Feature
Promega Corporation 1 2 3 4 5 Transcend™ Non-Radioactive Translation Detection System To verify this translation system is compatible with non-radioactive detection, linearized Luciferase T7 Control DNA * was titrated into the T N T ® T7 Quick for PCR DNA system in the presence of Transcend™ Biotinylated Lysine tRNA ( Cat.# L5061 ).
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Promega eNotes | Incite
Cells were permeabilized in a physiological buffer and allowed to extend nascent polypeptides by ~15 residues in the presence of tagged precursor molecules (either [ 3 H]lysine, biotin- or BODIPY-lys-tRNA), the remaining 19 amino acids, aminoacyl-tRNA synthetases, GTP and an energy generating system.
Thus, results with the FluoroTect™ GreenLys tag were essentially...
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Protein Expression Protocols and Applications Guide
Biotinylated lysine is added to the translation reaction as a pre-charged Iµ-labeled biotinylated lysine-tRNA complex (Transcenda„¢ tRNA) rather than a free amino acid.
Additional Resources for Transcenda„¢ Non-Radioactive Translation Detection Systems Technical Bulletins and Manuals TB182 Transcenda„¢ Non-Radioactive...
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Promega Product Profile - PROTEIN EXPRESSION & ANALYSIS
The system is based on a lysine-charged tRNA that is labeled at the ε position of the lysine with the fluorophore BODIPY ® -FL.
System Components Component Listing for L5001 1 x 40µl FluoroTect™; GreenLys tRNA Protocol Technical Bulletin #TB285 Figures Transcend tRNA and radiolabeled amino acids." title="View Figure 1"...
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Hampton Research
P12 - Screening organisms as important variable in the crystallization of the tRNA-modifying enzymes TGT and QueA Klaus Reuter, Clemens Grimm, and Ralf Ficner University Marburg Institute for Molecular Biology and Tumor Research, Emil-Mannkopff-Str., D-35037 Marburg, Germany The tRNA-modifying enzymes tRNA-guanine transglycosylase (TGT) and S-Adenosylmethionine-...
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Apoptosis Related Antibodies
-Product Size Catalog # Price *Anti - Keratin - 18 (Asp237), human -- NEW 250 µL 55148A view *Anti - Keratin - 19 (Asp237), human -- NEW 250 µL 55149A view Anti - AATF Apoptosis antagonizing transcription factor, Che - 1 50 µg 54124 view Anti - Acinus (CP) Apoptotic chromatin condensation inducer in the nucleus 50 µg 54057 view Anti - Acinus (CT) Apoptotic...
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S30 T7 High-Yield Protein Expression System Technical Manual #TM306
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author: Promega Corporation
subject: S30 T7 High-Yield Protein Expression System
updated: 22/08/2008
matching:
trna lysin 1
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S30 T7 High-Yield Protein Expression System Technical Manual #TM306 page 8
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author: Promega Corporation
subject: S30 T7 High-Yield Protein Expression System
updated: 22/08/2008
matching:
trna lysin 1
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