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Desalting/Buffer Exchange for Biomolecules Using AcroPrep 96 Ultrafiltration Filter Plates Protocol
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author: Ken
updated: 08/02/2006
matching:
factor 3a 2 66kda
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POLL Antibody POLL / BETA-N
Search ProSci Products -- new menu (MENU_ITEMS, MENU_POS); | ProSci | Products | Antibodies | Apoptosis Antibodies | Autophagy Antibodies | Cancer Antibodies | Chemokine Antibodies | Cytokine Antibodies | Growth Factor Antibodies | Homeostasis Antibodies | Immunology Antibodies | Infectious Disease Antibodies | Innate Immunity Antibodies | Monoclonal Antibodies | Neurobiology...
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Wnt Receptors and Pathways - R&D Systems
37 Wnts reported to activate Frizzled-1 include Wnt-1, 36 Wnt-8, 38 Wnt-3a, Wnt-3, and Wnt-2.
Canonical Wnts such as Wnt-1 and Wnt-3a block Strabismus binding of Dsh, and encourage its association with Frizzled.
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Citations Search
(1998) Transcript heterogeneity of the human reduced folate carrier results from the use of multiple promoters and variable splicing of alternative upstream exons.
Also, they cloned 3A´-UTR of this gene downstream of firefly luciferase coding region in pGL3-Control Vector to study posttranscriptional effects mediated by this region.
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Citations Search
(1998) Transcript heterogeneity of the human reduced folate carrier results from the use of multiple promoters and variable splicing of alternative upstream exons.
Also, they cloned 3A´-UTR of this gene downstream of firefly luciferase coding region in pGL3-Control Vector to study posttranscriptional effects mediated by this region.
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Citations Search
To screen miRNAs that regulate cyclin D1, the authors cloned the cyclin D1 3A´ untranslated region downstream of the firefly luciferase gene of the pGL3-Control Vector and measured luciferase levels in transfected cells using the Dual Luciferase Reporter Assay System.
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Citations Search
- Notes: The authors explored the role of five different eukaryotic initiation factor (eIF) 4GI protein isoforms, which are encoded by alternatively spliced mRNAs, by using short interfering RNAs (siRNAs) to silence the eIF4GI gene.
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Citations Search
A reporter vector was constructed using the core sequence of antioxidant reporter element (ARE) sythnesized by annealing two complementary oligonucleotides with Kpn1 and BglII at the 5a€™ and 3a€™ ends, respectively, and ligated into the same restriction sites of the pGL3-Promoter Vector (designated pGL3_ARE).
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Citations Search
- Notes: The authors explored the role of five different eukaryotic initiation factor (eIF) 4GI protein isoforms, which are encoded by alternatively spliced mRNAs, by using short interfering RNAs (siRNAs) to silence the eIF4GI gene.
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Citations Search
In this article, the authors explored the possibility that Brn-3b, which shares a POU domain 95% identical to Brn-3a, may interact with p53 and affect its role in apoptosis.
To examine the effect of Brn-3b on two p53-regulated genes, Bax and p21 cip1/waf1 , ND7 cells were transiently transfected with empty vector, Brn-3b, Brn-3a or p53, or Brn-3 with p53.
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Citations Search
To screen miRNAs that regulate cyclin D1, the authors cloned the cyclin D1 3A´ untranslated region downstream of the firefly luciferase gene of the pGL3-Control Vector and measured luciferase levels in transfected cells using the Dual Luciferase Reporter Assay System.
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Citations Search
- Notes: The authors explored the role of five different eukaryotic initiation factor (eIF) 4GI protein isoforms, which are encoded by alternatively spliced mRNAs, by using short interfering RNAs (siRNAs) to silence the eIF4GI gene.
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Citations Search
A reporter vector was constructed using the core sequence of antioxidant reporter element (ARE) sythnesized by annealing two complementary oligonucleotides with Kpn1 and BglII at the 5a€™ and 3a€™ ends, respectively, and ligated into the same restriction sites of the pGL3-Promoter Vector (designated pGL3_ARE).
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Citations Search
To determine whether the resulting TLS was regained by homologous or heterologous recombination with B1 or B2 RNA, the 3A´ end of the B3 RNA was amplified from leaf total RNA by RT-PCR using the AccessQuicka„¢ RT-PCR System, then sequenced.
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Citations Search
The 3A´ UTR containing the miRNA target site was cloned downstream of the luciferase gene in the pGL3 Promoter Vector and cotransfected into the miR-27b-positive breast cancer cell line MCF-7 and into miR-27b-negative Jurkat cells.
In this article, the authors explored the possibility that Brn-3b, which shares a POU domain 95% identical to Brn-3a, may interact...
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Citations Search
- Notes: The authors explored the role of five different eukaryotic initiation factor (eIF) 4GI protein isoforms, which are encoded by alternatively spliced mRNAs, by using short interfering RNAs (siRNAs) to silence the eIF4GI gene.
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Citations Search
Catalog --|-- Cart --|-- Log In Corporate Products Resources Search for Other Citations Sort By: Journal Year Items: 1-25 of 1304 - Next 1.
Reporter gene assays limit the production of luciferase by tying it to a promoter or DNA regulatory region of interest.
duplicates:
http://www.promega.com/citations/default.aspx?citapp=cell
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Citations Search
In this article, the authors explored the possibility that Brn-3b, which shares a POU domain 95% identical to Brn-3a, may interact with p53 and affect its role in apoptosis.
To examine the effect of Brn-3b on two p53-regulated genes, Bax and p21 cip1/waf1 , ND7 cells were transiently transfected with empty vector, Brn-3b, Brn-3a or p53, or Brn-3 with p53.
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Cloning Protocols and Applications Guide
Converting a 3A´ Overhang to a Blunt End Materials Required: Nuclease-Free Water ( Cat.# P1193 ) Bovine Serum Albumin (BSA), Acetylated, 1mg/ml ( Cat.# R9461 ) dNTPs, 100mM ( Cat.# U1240 ) T4 DNA Polymerase ( Cat.# M4211 ) T4 DNA Polymerase 10X buffer (optional) Note: T4 DNA Polymerase has a 3a€²a†’5a€² exonuclease activity that will, in...
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RNA Interference Protocols and Applications Guide
Combine each pair of DNA oligonucleotides to generate either the sense strand RNA or antisense strand RNA templates as follows: oligonucleotide 1 (100pmol/Aµl) 10Aµl oligonucleotide 2 (100pmol/Aµl) 10Aµl 2X oligo annealing buffer 50Aµl nuclease-free water 30Aµl Final Volume 100Aµl Heat at 90a€“95A°C for 3a€“5...
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FabGennix Inc. International >> Products
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FabGennix Inc. International >> Products
- -Search: - - - - - - - - - - - - - Antibodies We have following products/kits under category "Antibodies".
International.
International >> Products FabGennix Inc.
PDE assay kits, PDE4 subtype enzyme assay kit, Testicular Germ cell isolation kit, PDE4A8, PDE4A1, PDE4A5, PDE4A4, PDE4Ax, PDE1A, PDE1B, PDE1C, Antidepressant, Phospho-specific antibodies, ELISA, Western Blotting...
duplicates:
http://www.fabgennix.com/prod_category.asp?mycategory=Antibodies
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Cell Sciences Products
- Catalog No: - CRF018B --- (datasheet) Brand: - Cell Sciences Quantity: - 50 µg Price: - $315.00-- add-to-cart Category/Sub: - Proteins and Peptides / Recombinant Proteins Description: - Synonyms: Fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism), ACH, CEK2, FGFR-3, Fibroblast growth factor receptor 3 precursor, HSFGFR3EX, JTK4 Description...
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Cell Sciences Products
- Catalog No: - CRF018A --- (datasheet) Brand: - Cell Sciences Quantity: - 10 µg Price: - $250.00-- add-to-cart Category/Sub: - Proteins and Peptides / Recombinant Proteins Description: - Synonyms: Fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism), ACH, CEK2, FGFR-3, Fibroblast growth factor receptor 3 precursor, HSFGFR3EX, JTK4 Description...
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Cell Sciences Products
Product Search: or click here for advanced search: sFGFR-3/Fc Chimera, recombinant human - Catalog No: - CRF018B --- (datasheet) Brand: - Cell Sciences Quantity: - 50 µg Price: - $315.00-- add-to-cart Category/Sub: - Proteins and Peptides / Recombinant Proteins Description: - Synonyms: Fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism), ACH, CEK2,...
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