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Promega - FAQs
The -f'- indicates that the pGEM ® Vector includes a bacteriophage f1 origin of replication for the production of single-stranded DNA.
Vectors designated -(+)- or -(-)- differ only in the orientation of the f1 origin of replication and, therefore, which strand of DNA is packaged as single-stranded DNA.
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DNA Purification Protocols and Applications Guide
Plasmids derived from pUC contain a mutated version of the ColE1 origin of replication, which results in reduced replication control and approximately 200a€“700 plasmid copies per cell (high copy number).
The presence of the p15A origin of replication allows for replication of that particular plasmid in conjunction with a plasmid...
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Expression Analysis Protocols and Applications Guide
Some other factors to consider include: the amount of substrate available for labeling, its size in base pairs, the type of nucleic acid (DNA or RNA), the desired specific activity and whether it is double-stranded or single-stranded.
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Apoptosis Protocols and Applications Guide
Label DNA strand breaks with fluorescein-12-dUTP for 60 minutes at 37A°C in a humidified chamber protected from light.
Label DNA strand breaks with Biotinylated Nucleotide Mix (60 minutes at 37A°C).
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Nucleic Acid Amplification Protocols and Applications Guide
In the denaturation process, the two intertwined strands of DNA separate from one another, producing the necessary single-stranded DNA template for replication by the thermostable DNA polymerase.
PCR reagents can be treated with isopsoralen, a photo-activated, cross-linking reagent that intercalates into double-stranded DNA molecules and forms covalent,...
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Filtration & Separations Terminology
Recombinant DNA (rDNA) DNA formed by the joining of DNA strands into new combinations.
The strands of a DNA duplex, for example, are denatured at high temperatures but can be correctly reformed by slow cooling.
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Multi-well Filter Plate Glossary of Terms
-- DRUG CANDIDATE--Name given to a molecule discovered in R&D that elicits a desired biologic response.- After their discovery in R&D, drug candidates move down the drug discovery pipeline and are tested for ADMET properties and their ability to produce a desired biologic response in animal and human subjects.- -- DYE TERMINATOR--Used in DNA sequencing.- It is a fluorescently labeled...
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FAQs For IMPACT Protein Purification System, New England Biolabs
The vectors also contain the origin of DNA replication from bacteriophage M13, which allows for the production of single-stranded DNA by helper phage (M13KO7 helper phage, NEB #N0315) superinfection of cells bearing the plasmid.
The origin of DNA replication from bacteriophage M13, allows the production of single-stranded DNA by helper phage ...
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TJL BSS Panel: Chr 11 refs
Genetic mapping of 262 loci derived from expressed sequences in a murine interspecific cross using single-strand conformational polymorphism (SSCP) analysis.
Genetic mapping of 262 loci derived from expressed sequences in a murine interspecific cross using single-strand conformational polymorphism (SSCP) analysis.
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TJL BSS Panel: Chr 5 refs
Genetic mapping of 262 loci derived from expressed sequences in a murine interspecific cross using single-strand conformational polymorphism (SSCP) analysis.
Genetic mapping of 262 loci derived from expressed sequences in a murine interspecific cross using single-strand conformational polymorphism (SSCP) analysis.
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TJL BSS Panel: Chr 2 refs
Genetic mapping of 262 loci derived from expressed sequences in a murine interspecific cross using single-strand conformational polymorphism (SSCP) analysis.
Genetic mapping of 262 loci derived from expressed sequences in a murine interspecific cross using single-strand conformational polymorphism (SSCP) analysis.
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Hampton Research
In comparison to classical hammerhead structures, the folding domain found in STMV differs in the connection between the catalytic and the substrate strands.
The strand connections between the catalytic strand and the substrate strand are different and a conserved nucleotide, Cytosine (G384) is replaced by Guanosine.
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CLONTECHniques Archives- July 1996: New Living Colors (TM) GFP Bacterial Vectors
pBAD-GFPuv contains an M13 intragenic region for phage packaging and production of single-stranded DNA, and a Col E1 origin of replication and ampicillin resistance gene for propagation and selection, respectively, in E.
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CLONTECHniques Archives- October 1996: Great EscAPe (TM) SEAP Reporter System 2
The new vectors are each about 1.1 kb smaller than the original vectors and feature an expanded multiple cloning site (MCS).
The vector backbone also provides an f1 origin for single-stranded DNA production, and a pUC19 origin of replication and an ampicillin-resistance gene for propagation and selection in E.
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Clontech Laboratories, Inc. - Technical Info:
green fluorescent protein (ZsGreen) sequence: 628-1320 Mouse Ornithine Decarboxylase (MODC) d410 sequence: 1326-1485 SV40 early mRNA polyadenylation signal: 1638-1688 f1 single-strand DNA origin: 1735-2190 (Packages the noncoding strand of ZsGreen-MODC.) Bacterial promoter for expression of Kan r gene: -35 region: 2252-2257; -10 region: 2275-2280 Transcription start...
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Clontech Laboratories, Inc. - Technical Info: pDsRed2-N1 Vector
Red Fluorescent Protein (DsRed2) gene Kozak consensus translation initiation site: 672–682 Start codon (ATG): 679–681; Stop codon: 1354–1356 SV40 early mRNA polyadenylation signal Polyadenylation signals: 1508–1513 & 1537–1542; mRNA 3' ends: 1546 & 1558 f1 single-strand DNA origin: 1605–2060 (Packages the noncoding strand of DsRed2.)...
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Clontech Laboratories, Inc. - Technical Info: pDsRed2-C1 Vector
human codon-optimized Red Fluorescent Protein (DsRed2) gene Kozak consensus translation initiation site: 606–616 Start codon (ATG): 613–615; Stop codon: 1357–1359 Last amino acid in DsRed2: 1285–1287 MCS: 1288–1344 SV40 early mRNA polyadenylation signal Polyadenylation signals: 1498–1503 & 1527–1532; mRNA 3' ends: 1536 & 1648 f1 single-strand...
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Clontech Laboratories, Inc. - Technical Info: pDsRed2-1 Vector
human codon-optimized Red Fluorescent Protein (DsRed2) gene Kozak consensus translation initiation site: 90–100 Start codon (ATG): 97–99; Stop codon: 772–774 SV40 early mRNA polyadenylation signal Polyadenylation signals: 926–931 & 955–960 mRNA 3' ends: 964 & 976 f1 single-strand DNA origin: 1023–1478 (Packages noncoding strand of...
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HSV 1/2 DFA Kit, ~125 tests : 3290
- - Search: - About This Product Product Datasheet References Information Library MSDS 3290 ----3290 - LIGHT DIAGNOSTICS™ HSV DFA KIT CATALOG NUMBER: 3290 - LIST PRICE: Please click here to select your location, to view pricing - QUANTITY: 5 mL- - - EPITOPE: ~125 tests - DESCRIPTION: The Light Diagnostics HSV DFA is intended for the detection and identification of herpes simplex...
duplicates:
http://www.chemicon.com/Product/ProductDataSheet.asp?ProductItem=3290
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HSV 1&2; DFA Typing Kit, ~50 tests : 3291I
- - Search: - About This Product Product Datasheet Information Library MSDS 3291I ----3291I - LIGHT DIAGNOSTICS™ HERPES SIMPLEX VIRUS (HSV 1&2;) DFA TYPING KIT CATALOG NUMBER: 3291i - LIST PRICE: Please click here to select your location, to view pricing - QUANTITY: 2 mL each- - - EPITOPE: ~50 tests - DESCRIPTION: Intended Use The Light Diagnostics HSV 1/2 Typing DFA Kit is...
duplicates:
http://www.chemicon.com/Product/ProductDatasheet.asp?ProductItem=3291I
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Microbix - Newsroom - Glossary
The DNA molecule consists of four bases (adenine, cytosine, guanine and thymine) and a sugar-phosphate backbone, arranged in two connected strands to form a double helix.
Gene sequencing - Determination of the sequence of nucleotide bases in a strand of DNA.
Replication - Reproduction or duplication, as of an exact copy of a strand of DNA.
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HSV/VZV, ~125 tests : 3295
- - Search: - About This Product Product Datasheet References Images/Data Information Library MSDS 3295 ----3295 - LIGHT DIAGNOSTICS™ SIMULFLUOR® HSV/VZV KIT CATALOG NUMBER: 3295 - LIST PRICE: Please click here to select your location, to view pricing - QUANTITY: 5 mL- - - EPITOPE: ~125 tests - DESCRIPTION: The Light Diagnostics SimulFluor® HSV/VZV Immunofluorescence...
duplicates:
http://www.chemicon.com/Product/ProductDataSheet.asp?ProductItem=3295
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HSV 1/2, ~125 tests : 3293
- - Search: - About This Product Product Datasheet Images/Data Information Library MSDS 3293 ----3293 - LIGHT DIAGNOSTICS™ SIMULFLUOR® HSV 1/2 KIT CATALOG NUMBER: 3293 - LIST PRICE: Please click here to select your location, to view pricing - QUANTITY: 5 mL- - - EPITOPE: ~125 tests - DESCRIPTION: Light Diagnostics SimulFluor® HSV 1/2 Immunofluorescence Assay is a...
duplicates:
http://www.chemicon.com/Product/ProductDatasheet.asp?ProductItem=3293
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HSV/VZV, ~125 tests : 3295
They are large, enveloped viruses, about 190 nm in diameter containing linear, double-stranded DNA.
HSV infects mucocutaneous surfaces, then enters the dorsal root ganglia where further viral replication occurs, followed by a period of latency.
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HSV 1/2 DFA Kit, ~125 tests : 3290
They are large, enveloped viruses, about 190 nm in diameter containing linear, double-stranded DNA.
HSV infects mucocutaneous surfaces, then enters the dorsal root ganglia where further viral replication occurs, followed by a period of latency.
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