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Promega Notes Issue Index
Semi-Automated DNA Isolation and Analysis of GMO Foods 174kb Technically Speaking: Anti-ACTIVE ® Antibodies and MAPK Signaling Pathways 57kb - top of page - Issue 75 TITLE -PDF (size) Overview: High-Throughput Solutions 64kb Bright-Glo™ and Steady-Glo ® Luciferase Assay Systems: Reagents for Academic and Industrial Applications 261kb High-Throughput Solutions for DNA...
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Protein Expression Protocols and Applications Guide
Components [ 35 S] methionine Transcenda„¢ tRNA FluoroTecta„¢ GreenLys tRNA T N T A® Quick Master Mix 40Aµl 40Aµl 40Aµl Methionine, 1mM (mix gently prior to use) a€“ 1Aµl 1Aµl [ 35 S]methionine 1,000Ci/mmol at 10mCi/ml) 2Aµl a€“ a€“ PCR-generated DNA template 2.5a€“5Aµl 2.5a€“...
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Page: NAb Antibody Spin Columns
- Protein A Protein G Protein A/G Protein L Source Staphylococcus aureus Streptococci Bacillus Peptostreptococci MW 46,762 22,600 50,460 35,800 Number of IgG Binding Sites 4 2 4 4 Optimal Binding pH 7-8 5-8 5-8 7-7.5 Binds to IgG Fc Fc Fc VLk Related Products NAb Protein A Protein Columns and Kits NAb Protein G Protein Columns and Kits NAb Protein A/G Protein...
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Page: NAb Antibody Spin Columns
- Protein A Protein G Protein A/G Protein L Source Staphylococcus aureus Streptococci Bacillus Peptostreptococci MW 46,762 22,600 50,460 35,800 Number of IgG Binding Sites 4 2 4 4 Optimal Binding pH 7-8 5-8 5-8 7-7.5 Binds to IgG Fc Fc Fc VLk Related Products NAb Protein A Protein Columns and Kits NAb Protein G Protein Columns and Kits NAb Protein A/G Protein...
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Page: Comparison of Protein A, G, A/G and L
coli Molecular Weight 46,700 44,600 21,600 50,460 35,800 Apparent Mass by SDS-PAGE 42 kDa 45 kDa 32 kDa 40-45 kDa 36 kDa # Binding Sites for Ig 4 4 2 6 4 Albumin Binding Site No No No No No Optimal Binding pH 8.2 8.2 5 5-8.2 7.5 Ig Binding Target Fc Fc Fc Fc VL-kappa The four proteins bind almost exclusively with the IgG class of antibodies, but their...
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Page: Binding Capacity Tables
Recombinant Protein L** Native Protein A Recombinant Protein A Recombinant Protein G Protein A/G Source Peptostreptococci Staphylococcus aureus Bacilius Streptococci Bacilius Molecular Weight 35,8000 42,000 44,600 21,600 50,449 Number of Binding Sites for IgG 4 4 5 2 4 Albumin Binding Site no no no no no Optimal Binding pH 7.5 8.2 8.2 5 5-8.2 Optimal ...
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Page: Comparison of Protein A, G, A/G and L
coli Molecular Weight 46,700 44,600 21,600 50,460 35,800 Apparent Mass by SDS-PAGE 42 kDa 45 kDa 32 kDa 40-45 kDa 36 kDa # Binding Sites for Ig 4 4 2 6 4 Albumin Binding Site No No No No No Optimal Binding pH 8.2 8.2 5 5-8.2 7.5 Ig Binding Target Fc Fc Fc Fc VL-kappa The four proteins bind almost exclusively with the IgG class of antibodies, but their...
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NeutrAvidin Protein and Conjugates
- Avidin Streptavidin NeutrAvidin Protein Molecular Weight 67K 53K 60K Biotin-binding Sites 4 4 4 Isoelectric Point (pI) 10 6.8-7.5 6.3 Specificity Low High Highest Affinity for Biotin (Kd) 10 -15 M 10 -15 M 10 -15 M Nonspecific Binding High Low Lowest Applications for NeutrAvidin Protein: Western blotting – use NeutrAvidin Protein Conjugates (HRP or AP) instead of...
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Page: ELISA, pg 2
The graph represents cell proliferation assay results following a 24-hour cell incubation.- Blocking of Nonspecific Sites In an ELISA procedure, it is important to block the unoccupied sites on the surface of the well to reduce the amount of nonspecific binding of proteins during subsequent steps in the assay.
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Page: Binding Capacity Tables
Recombinant Protein L** Native Protein A Recombinant Protein A Recombinant Protein G Protein A/G Source Peptostreptococci Staphylococcus aureus Bacilius Streptococci Bacilius Molecular Weight 35,8000 42,000 44,600 21,600 50,449 Number of Binding Sites for IgG 4 4 5 2 4 Albumin Binding Site no no no no no Optimal Binding pH 7.5 8.2 8.2 5 5-8.2 Optimal ...
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NeutrAvidin Agarose and other NeutrAvidin Beads
- Biotin-Binding Protein Avidin Streptavidin NeutrAvidin Molecular Weight 67K 53K 60K Biotin-binding Sites 4 4 4 Isoelectric Point (pI) 10 6.8-7.5 6.3 Specificity Low High Highest Affinity for Biotin (Kd) 10 -15 M 10 -15 M 10 -15 M Nonspecific Binding High Low Lowest Highlights: Low nonspecific binding – Unlike avidin, NeutrAvidin Protein has no carbohydrates,...
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Label Transfer
Synthesis and Characterization of Insulin-like Growth Factor (IGF)-1 Photoprobes Selective for the IgG-Binding Proteins (IGFBPs); Photoaffinity Labeling of the IGF-Binding Domain on IGFBP-2.
SH3 Binding Sites of ZG29p Mediate an Interaction with Amylase and are Involved in Condensation-Sorting in the Exocrine Rat Pancreas.
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Proteomics - Label Transfer
Synthesis and Characterization of Insulin-like Growth Factor (IGF)-1 Photoprobes Selective for the IgG-Binding Proteins (IGFBPs); Photoaffinity Labeling of the IGF-Binding Domain on IGFBP-2.
SH3 Binding Sites of ZG29p Mediate an Interaction with Amylase and are Involved in Condensation-Sorting in the Exocrine Rat Pancreas.
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Proteomics - Western Blotting
Part-of-Thermo-Fisher-Scientific - Protein Structure- Introduction to Western Blotting Protein Transfer to a Membrane Blocking Nonspecific Binding Sites Washing Primary and Secondary Antibodies Antibody Labels Chromogenic Substrates Chemiluminescent Substrates Data Imaging Molecular Weight Markers Stripping and Reprobing a Membrane Related Techniques Troubleshooting...
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Proteomics - Immunohistochemistry
Part-of-Thermo-Fisher-Scientific - Protein Structure- Introduction to Immunohistochemistry Fixation of Tissues and Cells Inhibition of Endogenous Tissue Components Blocking of Nonspecific Sites Labeled Antibodies Avidin-Biotin Staining Immunohistochemical Substrates Immunohistochemical Troubleshooting Tech Tips Introduction to Immunohistochemistry Immunohistochemistry (IHC)...
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Page: ELISA, pg 2
The graph represents cell proliferation assay results following a 24-hour cell incubation.- Blocking of Nonspecific Sites In an ELISA procedure, it is important to block the unoccupied sites on the surface of the well to reduce the amount of nonspecific binding of proteins during subsequent steps in the assay.
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NeutrAvidin Agarose and other NeutrAvidin Beads
- Biotin-Binding Protein Avidin Streptavidin NeutrAvidin Molecular Weight 67K 53K 60K Biotin-binding Sites 4 4 4 Isoelectric Point (pI) 10 6.8-7.5 6.3 Specificity Low High Highest Affinity for Biotin (Kd) 10 -15 M 10 -15 M 10 -15 M Nonspecific Binding High Low Lowest Highlights: Low nonspecific binding – Unlike avidin, NeutrAvidin Protein has no carbohydrates,...
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NeutrAvidin Protein and Conjugates
- Avidin Streptavidin NeutrAvidin Protein Molecular Weight 67K 53K 60K Biotin-binding Sites 4 4 4 Isoelectric Point (pI) 10 6.8-7.5 6.3 Specificity Low High Highest Affinity for Biotin (Kd) 10 -15 M 10 -15 M 10 -15 M Nonspecific Binding High Low Lowest Applications for NeutrAvidin Protein: Western blotting – use NeutrAvidin Protein Conjugates (HRP or AP) instead of...
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Label Transfer
Synthesis and Characterization of Insulin-like Growth Factor (IGF)-1 Photoprobes Selective for the IgG-Binding Proteins (IGFBPs); Photoaffinity Labeling of the IGF-Binding Domain on IGFBP-2.
SH3 Binding Sites of ZG29p Mediate an Interaction with Amylase and are Involved in Condensation-Sorting in the Exocrine Rat Pancreas.
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Proteomics - Label Transfer
Synthesis and Characterization of Insulin-like Growth Factor (IGF)-1 Photoprobes Selective for the IgG-Binding Proteins (IGFBPs); Photoaffinity Labeling of the IGF-Binding Domain on IGFBP-2.
SH3 Binding Sites of ZG29p Mediate an Interaction with Amylase and are Involved in Condensation-Sorting in the Exocrine Rat Pancreas.
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Proteomics - Western Blotting
- Ordering Information Shipping Info Credit Info Returns Info - Current Order Currently Empty Order Form - YOU ARE HERE:- Protein Detection > Western Blotting Part-of-Thermo-Fisher-Scientific - Protein Structure- Introduction to Western Blotting Protein Transfer to a Membrane Blocking Nonspecific Binding Sites Washing Primary and Secondary Antibodies Antibody Labels...
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Proteomics - Immunohistochemistry
- Ordering Information Shipping Info Credit Info Returns Info - Current Order Currently Empty Order Form - YOU ARE HERE:- Protein Detection > Immunohistochemistry Part-of-Thermo-Fisher-Scientific - Protein Structure- Introduction to Immunohistochemistry Fixation of Tissues and Cells Inhibition of Endogenous Tissue Components Blocking of Nonspecific Sites Labeled Antibodies...
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Publications by NEB Scientists Before 2004, NEB
Identification of the Metal-Binding Sites of Restriction Endonucleases by Fe2+-Mediated Oxidative Cleavage.
Does Bcg1, a Unique Restriction Endonuclease, Require Two Recognition Sites for Cleavage?
A Structural Basis for the Preferential Binding of Hemimethylated DNA by HhaI DNA Methyltransferase.
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Histone Regulation
My Account | Order Status | Quick Order Products & Services Applications Nucleic Acid Purification & Analysis Nucleic Acid Amplification & Expression Profiling Cloning Proteins, Expression, Isolation & Analysis RNAi, Epigenetics & Gene Regulation Cell Culture Cell & Tissue Analysis Drug Discovery Clinical & Diagnostic Applications Bioproduction Products Antibodies & Secondary...
duplicates:
http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/RNAi-Epigenetics-and-Gene-Regulation/REGR-Misc/Epigenetics-Learning-Center/Histone-Regulation.html
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Interferon (IFN) Overview
My Account | Order Status | Quick Order Products & Services Applications Nucleic Acid Purification & Analysis Nucleic Acid Amplification & Expression Profiling Cloning Proteins, Expression, Isolation & Analysis RNAi, Epigenetics & Gene Regulation Cell Culture Cell & Tissue Analysis Drug Discovery Clinical & Diagnostic Applications Bioproduction Products Antibodies & Secondary...
duplicates:
http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-and-Tissue-Analysis/Signaling-Pathways/Interferon/Interferon-Overview.html
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