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Technical Notes: CCR7 - R&D Systems
6,7 Figure 1A & 1B.
The specificity of an anti-human CCR7 monoclonal ( Catalog # MAB197 ) was demonstrated by its ability to react with HEK-293 cells transfected with the human CCR7 receptor (A) and for its ability not to react with irrelevant transfectants (HEK-293 cells transfected with the human CCR4 gene) (B).
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New Antibodies :: ProSci Incorporated
Search ProSci Products -- new menu (MENU_ITEMS, MENU_POS); | ProSci | Products | Antibodies | Apoptosis Antibodies | Autophagy Antibodies | Cancer Antibodies | Chemokine Antibodies | Cytokine Antibodies | Growth Factor Antibodies | Homeostasis Antibodies | Immunology Antibodies | Infectious Disease Antibodies | Innate...
author: ProSci Inc.
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Enzymatic Protocol 2.0 - R&D Systems
After 4 hours, one can expect to see IL-1a, IL-1AŸ, IL-1ra, IL-2, IL-3, for various periods.
If cells are harvested after 3 hours, one can find monokines such as IL-1a, IL-1AŸ, IL-1ra, IL-6, IL-8, MIP-1a, MIP-1AŸ, and TNF-a.
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New Antibodies :: ProSci Incorporated
Search ProSci Products -- new menu (MENU_ITEMS, MENU_POS); | ProSci | Products | Antibodies | Apoptosis Antibodies | Autophagy Antibodies | Cancer Antibodies | Chemokine Antibodies | Cytokine Antibodies | Growth Factor Antibodies | Homeostasis Antibodies | Immunology Antibodies | Infectious Disease Antibodies | Innate...
author: ProSci Inc.
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Citations Search
- Notes: This paper describes an assay to test BDNF dissociation from FluoSpheres (amine-modified microspheres, 1I¼M diameter; Molecular Probes).- BDNF was covalently attached to the microspheres.- Release of BDNF-was tested in supernatants from the BDNF-microspheres in PBS,- in complete media, and-in culture with rat Dorsal root ganglia.- Promegaa€™s BDNF Emax A®...
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Citations Search
The authors used the Proteasome-Gloa„¢ Chymotrypsin-Like Cell-Based Assay to show that genotype-specific killing of "wound signature" cells was not due to differential degrees of proteasome inhibition, since 1 and 1Aµmol/L of bortezomib completely inhibited the ability of the wound signature and control cells to cleave the luminogenic substrate.
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Citations Search
They used a 1AµM ATP concentration and screened 55,000 compounds at 10AµM.
A total of 4pmol of PI3K/reaction was exposed to PDSP or a PI3K inhibitor followed by 25Aµg L-I±-phosphatidylinositol and 1AµM ATP before adding the Kinase-Glo A® Reagent.
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Citations Search
- Notes: This paper describes an assay to test BDNF dissociation from FluoSpheres (amine-modified microspheres, 1I¼M diameter; Molecular Probes).- BDNF was covalently attached to the microspheres.- Release of BDNF-was tested in supernatants from the BDNF-microspheres in PBS,- in complete media, and-in culture with rat Dorsal root ganglia.- Promegaa€™s BDNF Emax A®...
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Citations Search
The authors used the Proteasome-Gloa„¢ Chymotrypsin-Like Cell-Based Assay to show that genotype-specific killing of "wound signature" cells was not due to differential degrees of proteasome inhibition, since 1 and 1Aµmol/L of bortezomib completely inhibited the ability of the wound signature and control cells to cleave the luminogenic substrate.
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Citations Search
(2006) Acid-sensing ion channel 1a is a postsynaptic proton receptor that affects the density of dendritic spines.
They identified the acid-sensing ion channel 1a (ASIC1a) as a proton receptor, and they investigated its relationship to activation of the Ca 2+ /calmodulin-dependent protein kinase (CaM KII).
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New Antibodies :: ProSci Incorporated
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author: ProSci Inc.
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Protein Purification and Analysis Protocols and Applications Guide
Incubate for 1a€“2 minutes at room temperature.
In general a flow rate of 1a€“2ml/minute per milliliter of resin is optimal for efficient capture of polyhistidine-tagged protein.
If a second elution is desired, repeat Elution Steps 1a€“4.
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Cell Imaging Protocols and Applications Guide
Antibody Species (subclass) Application and Recommended Dilution 1 Known Species Cross-Reactivity Catalog Number Anti-Human BNDF pAb Chicken (IgY) Western 1I¼g/ml; ELISA 1I¼g/ml; ICC 1a€“10I¼g/ml; IHC 10a€“15I¼g/ml; BN 10I¼g/ml Human, mouse, rat, rabbit 2 and quail 2 Cat.# G1641 Anti-Rat CNTF pAb Chicken (IgY)...
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Apoptosis Protocols and Applications Guide
(2003) Oxygen regulated expression of the Wilms' tumor suppressor Wt1 involves hypoxia-inducible factor-1 (HIF-1).A-FASEB J.A-17, 1364a€“6.
During the final 1a€“2 hours of treatment, add 20I¼l/well of CellTiter-Blue A® Reagent directly to the culture wells.
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