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BDGI/OKL38: A New Breast Cancer Target - R&D Systems
When MCF-7 human breast adenocarcinoma cells are transfected with OKL38, in vitro cellular proliferation and in vivo tumor formation are reduced.
Further, BDGI overexpression in MCF-7 cells reduced their in vitro metastatic activity, arrested the cells in S phase, and induced apoptosis.
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Citations Search
- Notes: Transfectam A® Reagent was used to transfect 16 different cell lines (M413, 202, 151, 152, HeLa, A101, A549, MCF-7, EJ, SK-HEP-1, C2C12, THP macrophages, K562, Raji Burkitt lymphomas, LBW25 and LBW26 cells).
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Citations Search
- Notes: Transfectam A® Reagent was used to transfect 16 different cell lines (M413, 202, 151, 152, HeLa, A101, A549, MCF-7, EJ, SK-HEP-1, C2C12, THP macrophages, K562, Raji Burkitt lymphomas, LBW25 and LBW26 cells).
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Citations Search
One microgram of the various reporter constructs-along with 25ng pRL-null Vector (as an internal control) were transfected into HMEC-1 and MCF-7 cells in six-well plates.
(2002) NF-kB1 can inhibit v-Abl-induced lymphoid transformation by functioning as a negative regulator of cyclin D1 expression.
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Citations Search
One microgram of the various reporter constructs-along with 25ng pRL-null Vector (as an internal control) were transfected into HMEC-1 and MCF-7 cells in six-well plates.
- Notes: Drosophila S2 cells were transfected with a U6 maxigene plasmid and a control firefly luciferase construct (derived from the pGL2 Basic Vector) using the ProFection A®...
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Citations Search
Neomycin resistant stable cell lines derived from U251 human glioma cells expressing luciferase under the control of either the HSV thymidine kinase (TK) with-or without hypoxia-response element (HRE) repeats, or the SV40 promoter were constructed.- U251 cell lines expressing luciferase under control of either a vascular endothelial growth factor ...
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Citations Search
- Notes: These authors created a construct encoding a Renilla and firefly luciferase fusion protein to examine the efficiency of site-specific DNA repair.-The Renilla gene was taken from the pRL-null Vector and the firefly luciferase gene originated from the pSP-luc+NF Fusion Vector.-The fusion construct was created by ligating the C-terminus of the Renilla luciferase gene to the N...
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Citations Search
One microgram of the various reporter constructs-along with 25ng pRL-null Vector (as an internal control) were transfected into HMEC-1 and MCF-7 cells in six-well plates.
(2002) NF-kB1 can inhibit v-Abl-induced lymphoid transformation by functioning as a negative regulator of cyclin D1 expression.
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Citations Search
One microgram of the various reporter constructs-along with 25ng pRL-null Vector (as an internal control) were transfected into HMEC-1 and MCF-7 cells in six-well plates.
- Notes: Drosophila S2 cells were transfected with a U6 maxigene plasmid and a control firefly luciferase construct (derived from the pGL2 Basic Vector) using the ProFection A®...
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Citations Search
Neomycin resistant stable cell lines derived from U251 human glioma cells expressing luciferase under the control of either the HSV thymidine kinase (TK) with-or without hypoxia-response element (HRE) repeats, or the SV40 promoter were constructed.- U251 cell lines expressing luciferase under control of either a vascular endothelial growth factor ...
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Citations Search
Transformation of the luciferase constructs into the breast cancer cell lines, MCF-7 and MDA-MB-231, both of which expressed high levels of miR-206 as measured by real-time PCR, resulted in repression of luciferase activity.
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Citations Search
The 3A´ UTR containing the miRNA target site was cloned downstream of the luciferase gene in the pGL3 Promoter Vector and cotransfected into the miR-27b-positive breast cancer cell line MCF-7 and into miR-27b-negative Jurkat cells.
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Citations Search
Transformation of the luciferase constructs into the breast cancer cell lines, MCF-7 and MDA-MB-231, both of which expressed high levels of miR-206 as measured by real-time PCR, resulted in repression of luciferase activity.
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Citations Search
- Notes: These authors created a construct encoding a Renilla and firefly luciferase fusion protein to examine the efficiency of site-specific DNA repair.-The Renilla gene was taken from the pRL-null Vector and the firefly luciferase gene originated from the pSP-luc+NF Fusion Vector.-The fusion construct was created by ligating the C-terminus of the Renilla luciferase gene to the N...
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Citations Search
Transformation of the luciferase constructs into the breast cancer cell lines, MCF-7 and MDA-MB-231, both of which expressed high levels of miR-206 as measured by real-time PCR, resulted in repression of luciferase activity.
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Citations Search
The 3A´ UTR containing the miRNA target site was cloned downstream of the luciferase gene in the pGL3 Promoter Vector and cotransfected into the miR-27b-positive breast cancer cell line MCF-7 and into miR-27b-negative Jurkat cells.
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Citations Search
Transformation of the luciferase constructs into the breast cancer cell lines, MCF-7 and MDA-MB-231, both of which expressed high levels of miR-206 as measured by real-time PCR, resulted in repression of luciferase activity.
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Citations Search
Transformation of the luciferase constructs into the breast cancer cell lines, MCF-7 and MDA-MB-231, both of which expressed high levels of miR-206 as measured by real-time PCR, resulted in repression of luciferase activity.
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Transfection Protocols and Applications Guide
Various allelic sequences were then cloned into a modified phRL-SV40 Renilla luciferase reporter vector and transfected into the mouse SN4741 cell line, which expresses the dopamine transporter, and the effects on reporter activity were monitored.
(2004) Cyr61 expression confers resistance to apoptosis in breast cancer MCF-7 cells by a mechanism of NF...
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Bioluminescence Reporters Protocols and Applications Guide
Additional Resources for Live Cell Substrates Technical Bulletins and Manuals TB362 GloResponsea„¢ CRE-luc2P HEK293 Cell Line Technical Bulletin TB363 GloResponsea„¢ NFAT-RE-luc2P HEK293 Cell Line Technical Bulletin Selecting a Reporter Gene and Assay: Tables 8.1a€“8.3 The tables in this section are designed to show the...
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Transfection Selection Tool
Plasmid DNA Lipofectamine™ LTX and PLUS™ Reagent 15338-100 15338-500 11514-015 LNCap Human prostate cancer Plasmid DNA Lipofectamine™ LTX and PLUS™ Reagent 15338-100 15338-500 11514-015 LS180 Human colon carcinoma siRNA Lipofectamine™ RNAiMAX 13778-075 13778-150 - LSMC Human leiomyoma smooth muscle cells- siRNA Lipofectamine™ RNAiMAX 13778...
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Probes Online - September 2008, Volume 11, Number 8
My Account | Order Status | Quick Order Products & Services Applications Nucleic Acid Purification & Analysis Nucleic Acid Amplification & Expression Profiling Cloning Proteins, Expression, Isolation & Analysis RNAi, Epigenetics & Gene Regulation Cell Culture Cell & Tissue Analysis Drug Discovery Clinical & Diagnostic Applications Bioproduction Products Antibodies & Secondary...
duplicates:
http://www.invitrogen.com/site/us/en/home/support/Newsletters-and-Journals/ProbesOnline/Probes-Online-September-2008-Volume-11-Number-8.html
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Transfection Selection Tool
Plasmid DNA Lipofectamine™ LTX and PLUS™ Reagent 15338-100 15338-500 11514-015 LNCap Human prostate cancer Plasmid DNA Lipofectamine™ LTX and PLUS™ Reagent 15338-100 15338-500 11514-015 LS180 Human colon carcinoma siRNA Lipofectamine™ RNAiMAX 13778-075 13778-150 - LSMC Human leiomyoma smooth muscle cells- siRNA Lipofectamine™ RNAiMAX 13778...
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Quantikine BMP-2 ELISA
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author: Alan
updated: 30/10/2008
matching:
mcf cell line deriv transform sequenc
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MCF2/Dbl Antibody #2089
-|- contact -|- browsers & accessibility -|- careers MCF2/Dbl Antibody #2089 MCF2/Dbl Antibody #2089 MCF MCF 2 MCF 2 CELL LINE-DERIVED TRANSFORMING SEQUENCE MCF-2 MCF2 MCF2 DBL ANTIBODY P P 10911 P-10911 P10911 MCF MCF 2 MCF 2 CELL LINE-DERIVED TRANSFORMING SEQUENCE MCF-2...
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