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Development of Somitic Lineages Poster References, May 2006 - R&D Systems
"Maintenance of somite borders in mice requires the Delta homologue DII1." Nature386(6626): 717-21.
"Conserved and divergent roles for members of the Snail family of transcription factors in the chick and mouse embryo." Development125(16): 3111-21.
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Bone Morphogenetic Proteins (BMPs) - R&D Systems
20, 21 This complex is secreted.
Type II receptors have a 21 aa cytoplasmic C-terminal extension which, although capable of being phosphorylated, is not necessary for activity.
74, 75 Type I Receptors ALK-1: ALK-1 (Activin-like kinase-1) is a 62-72 kDa, 503 aa type I transmembrane glycoprotein that contains a 21 aa signal sequence, a 97 aa extracellular region...
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Citations Search
(1997) A type I interferon signaling factor, ISF21, encoded on chromosome 21 is distinct from receptor components and their down-regulation and is necessary for transcriptional activation of interferon-regulated genes.
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Citations Search
Experimental pGL3 Basic vectors were cotransfected with pRL-TK at a 2:1 ratio.
One of the pGL3 Basic Vector-based constructs had the PEPCK promoter plus a GAL4 binding domain and another had five tandem repeats of the GAL4 domain and a minimal TATA box.
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Citations Search
- Notes: The Y-box-binding protein YB-1 was examined through the use of a reporter plasmid with wildtype and mutant putative Y-box binding sites.-The binding sites were constructed in the-pGL3 Basic Vector and measured using the Luciferase Assay System.
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Citations Search
GC boxes are essential for cell cycle-regulated transcription of the cyclin A1 gene.
Experimental pGL3 Basic vectors were cotransfected with pRL-TK at a 2:1 ratio.
One of the pGL3 Basic Vector-based constructs had the PEPCK promoter plus a GAL4 binding domain and another had five tandem repeats of the GAL4 domain and a minimal TATA box.
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Citations Search
85(Pt 7) , 2111-21.
(2004) Efficient prenylation by a plant geranylgeranyltransferase-I requires a functional CaaL box motif and a proximal polybasic domain.
NeuschA¤fer-Rube, F., Engemaier, E., Koch, S., BA¶er U., and PA¼schel, G.P.
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Citations Search
(1997) A type I interferon signaling factor, ISF21, encoded on chromosome 21 is distinct from receptor components and their down-regulation and is necessary for transcriptional activation of interferon-regulated genes.
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Citations Search
- Notes: The Y-box-binding protein YB-1 was examined through the use of a reporter plasmid with wildtype and mutant putative Y-box binding sites.-The binding sites were constructed in the-pGL3 Basic Vector and measured using the Luciferase Assay System.
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Citations Search
Experimental pGL3 Basic vectors were cotransfected with pRL-TK at a 2:1 ratio.
One of the pGL3 Basic Vector-based constructs had the PEPCK promoter plus a GAL4 binding domain and another had five tandem repeats of the GAL4 domain and a minimal TATA box.
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Citations Search
- Notes: The Y-box-binding protein YB-1 was examined through the use of a reporter plasmid with wildtype and mutant putative Y-box binding sites.-The binding sites were constructed in the-pGL3 Basic Vector and measured using the Luciferase Assay System.
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Citations Search
Tissue-specific expression of the 1Ax1 gene, which has an endosperm-specific promoter, was examined using RT-PCR in 21 transgenic wheat lines.
IL-6, NF-AT, p53, NF-IºB, Ap-1 and cAMP response elements were cloned upstream of a TATA box and the firefly luciferase reporter gene.
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Citations Search
GC boxes are essential for cell cycle-regulated transcription of the cyclin A1 gene.
Experimental pGL3 Basic vectors were cotransfected with pRL-TK at a 2:1 ratio.
One of the pGL3 Basic Vector-based constructs had the PEPCK promoter plus a GAL4 binding domain and another had five tandem repeats of the GAL4 domain and a minimal TATA box.
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Citations Search
21 , 4960-4967.
After verification by sequencing, the promoter and enhancer were cloned individually and together into the pGL3-Basic Vector.-Additionally, an Xho I/Sal I fragment containing the HSV tk promoter, a gene conferring neomycin resistance, and a poly-A tail were cloned into the Sal I site of pGL3 to allow the production of stable transfectants.-To perform stable...
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Citations Search
85(Pt 7) , 2111-21.
(2004) Efficient prenylation by a plant geranylgeranyltransferase-I requires a functional CaaL box motif and a proximal polybasic domain.
NeuschA¤fer-Rube, F., Engemaier, E., Koch, S., BA¶er U., and PA¼schel, G.P.
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Citations Search
- Notes: The Y-box-binding protein YB-1 was examined through the use of a reporter plasmid with wildtype and mutant putative Y-box binding sites.-The binding sites were constructed in the-pGL3 Basic Vector and measured using the Luciferase Assay System.
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Citations Search
- Notes: The Y-box-binding protein YB-1 was examined through the use of a reporter plasmid with wildtype and mutant putative Y-box binding sites.-The binding sites were constructed in the-pGL3 Basic Vector and measured using the Luciferase Assay System.
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Citations Search
- Notes: The pGL3-Control Vector was co-transfected into COS-7 cells with mammalian expression vectors expressing either heat shock protein Hsp70 or Hsp105α.- The cells were ATP-depleted in glucose-free media with 2μM rotenone and 5mM 2-deoxyglucose for up to 3 hours.-Afterwards the cells were lysed with the Cell Culture Lysis Reagent and assayed for luciferase...
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Citations Search
- Notes: To further understand the transcription factor paired-box 2 ( PAX2) gene, 2Aµg of total RNA isolated from Kaposia€™s sarcoma cells were reverse transcribed, and 5Aµl of the RT reaction was amplified.
(0001303) - - Products: pGEM ® -T Vector System I | pGEM ® -T Vector System II | Prime-a-Gene ® Labeling System | pT ARGE T™...
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Citations Search
(An improved version of the pCAT A® - Basic Vector, the pCAT A® -3 Basic Vector is now available) (0001466) - - Products: Erase-a-Base ® System (minus vectors & bacterial strain) | pCAT ® 3-Basic Vector 21.
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Citations Search
Tissue-specific expression of the 1Ax1 gene, which has an endosperm-specific promoter, was examined using RT-PCR in 21 transgenic wheat lines.
IL-6, NF-AT, p53, NF-IºB, Ap-1 and cAMP response elements were cloned upstream of a TATA box and the firefly luciferase reporter gene.
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Citations Search
21 , 4960-4967.
After verification by sequencing, the promoter and enhancer were cloned individually and together into the pGL3-Basic Vector.-Additionally, an Xho I/Sal I fragment containing the HSV tk promoter, a gene conferring neomycin resistance, and a poly-A tail were cloned into the Sal I site of pGL3 to allow the production of stable transfectants.-To perform stable...
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Citations Search
- Notes: The Y-box-binding protein YB-1 was examined through the use of a reporter plasmid with wildtype and mutant putative Y-box binding sites.-The binding sites were constructed in the-pGL3 Basic Vector and measured using the Luciferase Assay System.
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Citations Search
(0003597) - - Products: Dual-Luciferase ® Reporter 1000 Assay System | Dual-Luciferase ® Reporter Assay System | Dual-Luciferase ® Reporter Assay System 10-Pack | pGL4.74[hRluc/TK] Vector | pRL-TK Vector | T N T ® T7 Coupled Reticulocyte Lysate System | T N T ® T7 Coupled Reticulocyte Lysate System, Trial Size | T N T ® T7 Quick Coupled Transcription...
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Citations Search
- Notes: To determine the potential role that T-bet, a T-box transcription factor that specifies Th1 lineage commitment, and Hlx, a homeobox gene, may have in helper T cell differentiation, the physical interaction between the two proteins was tested using the CheckMatea„¢ Mammalian Two-Hybrid System.
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